WebAug 11, 2011 · ChimeraScan includes features such as the ability to process long (>75 bp) paired-end reads, processing of ambiguously mapping reads, detection of reads spanning a fusion junction, integration with the popular Bowtie aligner ( Langmead et al., 2009 ), supports the standardized SAM format and generation of HTML reports for easy … WebOct 6, 2015 · 131.4 Gb pair-end (PE) sequence data was reanalyzed for the chimeras. Totally, 40,259,438 read pairs (6.19%) with chimerism were discovered among 650,430,811 read pairs. The chimeric sequences are consisted of two or more parts which locate inconsecutively but adjacently on the chromosome.
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Chimera (molecular biology) - Wikipedia
WebNov 12, 2024 · Chimeric reads are significantly more abundant in sequencing data from FFPE genome libraries Sequencing from both ends of a library insert, referred to as paired-end sequencing (PE-seq), is often the preferred sequencing approach in NGS analyses. WebAug 16, 2024 · The long-read nature of nanopore sequencing presents an effective tool for the discovery and filtering of chimeric reads. We have found that at least 1.7% of reads prepared using the Nanopore ... WebJul 30, 2024 · Gene fusion, trans-splicing or transcription read-through contributes to generation of chimeric RNA. Here the authors develop a pipeline to identify non-canonical type of chimeric RNAs called ... iphone 12 pro max case card holder