Gold antifade reagent
WebI have used Prolong Gold Antifade with DAPI on cytospun cells or frozen human skeletal muscle tissue sections, 5 µm thick, fixed with either acetone or formaldehyde w/ or w/o triton-x as... WebDec 31, 2024 · Cells were then washed twice with HBSS and fixed using 2% paraformaldehyde-HBSS for 15 min at 37 °C. Fixed cells were washed three times with HBSS, and coverslips were mounted onto glass slides using ProLong Gold Antifade reagent (Invitrogen, Australia). Coverslips were examined using a Leica confocal …
Gold antifade reagent
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Web" The ProLong Gold Antifade Mountant is a ready to use mountant liquid that can be directly applied to fluorescently labelled cell or tissue samples on microscope slides. The best feature is that cures for longer-term storage and samples are stable for months. There is little quenching but it's almost undetectable. http://formedium.equl.cn/goods.php?id=4907
WebThe City of Fawn Creek is located in the State of Kansas. Find directions to Fawn Creek, browse local businesses, landmarks, get current traffic estimates, road conditions, and … WebProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, add a coverslip, cure, and image. Supplier: Invitrogen™ P36930
WebMar 20, 2008 · The SlowFade® Gold reagent is provided as a premixed and ready-to-use solution. Only 1-2 drops of antifade are required per section. After applying SlowFade® … WebJan 17, 2024 · For confocal microscopic analysis, antibodies stained samples were mounted with fluorescence mounting medium (ProLong Gold Antifade Reagent, ThermoFisher Scientific, OR, USA) and images were obtained with a FV1000 or FV3000 laser-scanning confocal microscope (Olympus, Tokyo, Japan). ASW2.1 and FV31S software (Olympus) …
WebDec 11, 2024 · The cells were rinsed, and then, coverslips were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, Danvers, MA, USA, catalog #8961S) and incubated overnight at 4 °C. Images were taken with an Olympus Fluoview 3000 confocal microscope and processed using the Olympus Fluoview FV10-ASW 4.1 …
WebAntifade Reagents provide protection against fading or photobleaching for most common fluorophores used in live and fixed cell fluorescent imaging. Products. Background. Literature (1) Cat. No. Product Name / Activity. 4055. L -Ascorbic acid. Commonly used antifade reagent in live cell microscopy; naturally occurring antioxidant. heng hiap kempasWebThe only advantage of using the Prolong gold antifade mounting reagent with DAPI is to save you the pain of adding DAPI with your secondary antibodies... So the advantage is quite limited. If... heng hong tin kee restaurant melakaWebJun 13, 2006 · ProLong® Gold antifade reagent is a ready-to-use mounting medium intended for use with fluorescent imaging techniques. To prepare your sample, you only have to put a drop of this reagent onto your … heng hiap metal sdn bhdWebSamples were mounted in ProLong ® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product. Show Less Show More. Confocal immunofluorescent analysis of mouse retina using Neurogranin (E9L8D) Rabbit mAb (green), GFAP (GA5) Mouse mAb (Alexa Fluor ... evercoss kamera terbalikWebSome drug abuse treatments are a month long, but many can last weeks longer. Some drug abuse rehabs can last six months or longer. At Your First Step, we can help you to … evercraft 7769250 amazonWebDescription ProLong Gold Antifade Mountant with DNA Stain DAPI is a mounting media that can be directly applied to fluorescently labeled cell or tissue samples on microscope slides. It comes ready-to-use and stored at room temperature—just apply a drop, add a coverslip, cure, and image. heng heng raja udaWebSlowFade Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It … heng hua mee sua