Pmutin-gfp+
WebJun 25, 2015 · Plasmid pMutin-GFP+ contains a gfp reporter with three terminators (t1, t2, t0 from the rrnB operon of E. coli ) upstream the multiple cloning site in front of gfp and a trpA terminator downstream of gfp . pMutin-GFP+ was amplified with primers PG187 and PG188 in order to remove the Pspac promoter and to introduce 5 unique restriction sites in ... WebThe homologous recombination fragment was inserted into the integration vector pMUTIN-GFP+, which could integrate into chromosome by a single recombination event, to construct knockout plasmid.
Pmutin-gfp+
Did you know?
WebJan 29, 2016 · Two separate plasmids namely pMUTIN-GFP+ and pDG1662 were used for the construction of plasmid pDG1662-A. On isolation of genomic DNA from B. subtilis … Web你好 师姐 你有pmutin质粒吗？ 师妹最近也在做芽孢的敲除实验 但是一直做了4个月了 都毫无收获 现在已经研三了 可以把你的质粒卖给我吗 谢谢 赞 一下
WebDec 1, 1999 · GFP+ and CD4+8+ immature T-cells developed normally from bone marrow cells in the reconstituted thymus. The experimental system using hematopoietic cells … WebJan 1, 2016 · Transformants were PCR confirmed using pMUTIN-GFP gene specific primers ( Table S1) that were designed from gfp gene sequences for amplification of 359 bp. PCR was performed in GoTaq PCR kit of Promega (GoTaq Buffer-1X, MgCl 2 – 1.5 mmol l −1, dNTPs – 200 μmol l −1, Forward/Reverse primers – 10 pmol each, DMSO – 6%, Taq …
WebFeb 20, 2009 · Europe PMC is an archive of life sciences journal literature. Webits promoter from pXX167 (17) into the Clal site of pMUTIN-GFP+ (46). This construction gave rise both to a wild-type SopA-GFP plasmid and to another plasmid that had two spon …
WebpMUTIN-GFP+ Erythromycin selectable in EC or BS; Ampicillin selectable only in EC; GFP+ full coding sequence, including RBS and Pspac; IPTG-inducible; full plasmid insertion; ECE149 Map. From Bayreuth: No: 3 bands on the gel ECE150 pMUTIN-CFP Erythromycin selectable in EC or BS;
WebApr 8, 2005 · Plasmid pMUTIN-GFP+ is a cloning vector (based on a ColE1-like replicon) designed for construction and expression of fusion genes encoding hybrid proteins composed of N-terminal part of the desired protein (or. PAP I leader sequence directs the protein to cellular membrane. red gaming background strixhttp://muchong.com/t-5006068-2 knot\u0026ropesupplyWebHD-73 strain of Bacillus thuringiensis transformed with the pMUTIN-gfp vector, containing the gpf gene under the control of the pspac promoter (Bt HD73/pMUTIN-gfp). (a, c and e) Phase contrast ... red gamer pictureWebpMUTIN-GFP+ Erythromycin selectable in EC or BS; Ampicillin selectable only in EC; GFP+ full coding sequence, including RBS and Pspac; IPTG-inducible; full plasmid insertion knot4xWebFeb 3, 2011 · For FACS experiments performed 7–14 days after muscle dissociation, cultured nestin-GFP+ cells were washed with phosphate-buffered saline (PBS) and treated with 0.25% trypsin/0.05% EDTA to isolate them in a suspension. When all cells were detached, the enzymatic reaction was stopped with the resuspension medium described … knot-tying wordsWebDownload scientific diagram HD-73 strain of Bacillus thuringiensis transformed with the pMUTIN-gfp vector, containing the gpf gene under the control of the pspac promoter (Bt … red gaming banner art templateknot204b